We developed a method for direct real-time measurement of biomolecular responses and interactions using the microfluidic probe technology. Leveraging segmented flow to preserve sharp interfaces between sequentially injected reagents allows maintaining a desired initial concentration of the reactant on the reaction surface, and thus enables an accurate characterization of the surface-reaction.

The contact-free operation of thec MFP prevents perturbation of the sample on the surface and allows addressing multiple reaction sites on the surface automatically, opening the door to multiplexed measurements of reaction kinetics.

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This work is done within the framework of the EU-Virtual Vials project with the Microfluidic Technologies group at Technion – Israel Institute of Technology.

nadyaBiomolecular Responses