Cellular heterogeneity is imperative for healthy functioning of tissues but when uncontrolled, it is implicated in disease development and progression. In the context of cancer, this heterogeneity complicates an accurate diagnosis. Traditionally, tumor analysis is performed on blocks from biopsies or whole tumor sections, which contain cell populations of interest, diluted with non-malignant cells. Further aggravating the analysis is the scarcity of extracted biopsies, allowing only a few tests per sample. Therefore, it is essential to realize methods that locally and accurately probe molecular characteristics of these samples, while efficiently budgeting the biopsy sample. We are developing a range of methods to perform histochemistry and immunohistochemistry at the micrometer-scale on tissue sections for comprehensive local profiling of tissues at the genomic and transcriptomic level. We term such processing of tissue sections at the micrometer-scale tissue microprocessing.
Hematoxylin staining on a cell-block section
Local hematoxylin staining on an eosin counterstained cell-block section using the probe technology.